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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 150,487 results for: ins

Showing: 3001 - 3020 of 150487 results

  1. pFastBacDual with LFn + Needle

    Plasmid
    #84868
    Purpose
    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Needle
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84868-plasmid-map-sequence-id-162901

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  3. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  5. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172832-plasmid-map-sequence-id-340914

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  10. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid
    #172828
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172828-plasmid-map-sequence-id-341092

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  11. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  19. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

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Showing: 3001 - 3020 of 150487 results