We narrowed to 157,746 results for: ins
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Plasmid#175829PurposeExpress mCherry with microtubule-targeting sequence in mammalian cellsDepositorInsertmCherry with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-mKate2
Plasmid#48345PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway CloningMutationContains a stop codon at the 3' end of the c…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-EPSIN 2
Plasmid#22242DepositorAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha pRC/CMV
Plasmid#8690DepositorInsertSTAT1 alpha (STAT1 Human)
ExpressionMammalianAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-EGFP
Plasmid#175827PurposeExpress EGFP with microtubule-targeting sequence in mammalian cellsDepositorInsertEGFP with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-EGFP
Plasmid#14121DepositorAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-hKCNE1
Plasmid#53050PurposeExpresses beta subunit for IKs channel in mammalian cellsDepositorInsertPotassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
ExpressionMammalianPromoterCMVAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hKCNE1
Plasmid#53049PurposeExpresses beta subunit for IKs channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
UseXenopus oocyte expressionPromoterSP6Available SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EYFP
Plasmid#48351PurposeContributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway CloningMutationContains a stop codon.PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN477
Plasmid#86232PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-EEA1
Plasmid#174453PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab11
Plasmid#174462PurposeExpress iRFP with recycling endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab5
Plasmid#174456PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
Tagssmall GTPase (Rab5)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only