We narrowed to 31,396 results for: Eng

1. Tol2_8xCTS-ECFP

   Plasmid

   #200249

   Purpose

   Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   8xCTS-ECFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ147-BvCas12b-sgRNA-scaffold

   Plasmid

   #122447

   Purpose

   Expresses sgRNA for BvCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP_sfCherry2(11)_zyxin

   Plasmid

   #83034

   Purpose

   Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_zyxin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. JP1520 PIK3CA E545K HA

   Plasmid

   #14571

   Depositor

   Joan Brugge

   Article

   Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

   Insert

   PIK3CA E545K (PIK3CA Bovine)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   E545K

   Promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pICSL70008

   Plasmid

   #50336

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWH-DI

   Plasmid

   #47385

   Purpose

   Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   dxs
   idi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PxylA

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pICSL70004

   Plasmid

   #50334

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCV-HF

   Plasmid

   #32057

   Depositor

   Patrick Moore

   Article

   Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

   Insert

   MCV-HF genome
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none from vector. Insert contains native promoter…

   Available since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-U6a

   Plasmid

   #107593

   Purpose

   An entry vector with U6a promoter driving guide RNA expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   U6a promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK_Sox9_enh_366_Citrine

    Plasmid

    #130593

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox9_enh_366
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK_Sox9_enh_283_Citrine

    Plasmid

    #130591

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox9_enh_283
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis8-4b-SaDAH

    Plasmid

    #140128

    Purpose

    Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Sinomenium acutum
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Promoter

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORF1

    Plasmid

    #206025

    Purpose

    An expression vector for mouse LINE1 ORF1

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1 derived from mouse LINE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMZ2007

    Plasmid

    #204399

    Purpose

    A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

    Inserts

    quorum sensing regulator LuxR
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PftsH and Synthetic promoter PLux0002

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pre_II_CS3_7560_v1

    Plasmid

    #126853

    Purpose

    7560 b scaffold from compatibility group 3, made by method II

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7560_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMYs-IRES-mitoRFP

    Plasmid

    #121996

    Purpose

    Expression of mitoRFP in mammalian cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    IRES-mitoRFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pILGFP4M

    Plasmid

    #104508

    Purpose

    Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SeGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTK_Snai2_enh_13_Citrine

    Plasmid

    #130574

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Snai2_enh_13
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAcGP67A human LRP6 E1E2 (20–629)

    Plasmid

    #132627

    Purpose

    expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–629)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. tGFP

    Plasmid

    #26864

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    Truncated GFP-IRES-Puro
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of nt 1-36

    Promoter

    Available since

    Nov. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pICH87633

    Plasmid

    #50271

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p70 delta2-46 delta CT104

    Plasmid

    #1898

    Depositor

    Joseph Avruch

    Article

    Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

    Insert

    p70 alpha1 S6 kinase (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta2-46, deltaCT104 (deletion of aa 2-46 and am…

    Promoter

    Available since

    March 17, 2006

    Availability

    Academic Institutions and Nonprofits only

27. JP1520 PIK3CA H1047R HA

    Plasmid

    #14572

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA H1047R (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pre_IV_RFP_1317_v1

    Plasmid

    #126863

    Purpose

    1317 b scaffold based on pSB1A3 backbone with RFP gene as insert

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_RFP_1317_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pGGF002

    Plasmid

    #48843

    Purpose

    Provides a BASTA resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    p35S::BastaR::t35S
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    internal BsaI recognition site in 35S promoter re…

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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