We narrowed to 8,385 results for: Dos

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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61426-hU6-shmFmr1-EF1a-mCherry

Plasmid

#157859

Purpose

Expresses a shRNA targeting mouse Fmr1 gene

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Fmr1 (Fmr1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Triple ORF LoxP ifgMosaic Donor (LH208)

Plasmid

#99625

Purpose

To clone the 3 ORFs for Cre dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MEK1-pcw107-V5

Plasmid

#64650

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T12/35A

Plasmid

#47938

Purpose

expresses Myc tagged human MOB1 containing T12A and T35A mutations

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Tags

Myc

Expression

Mammalian

Mutation

T12A and T35A

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
CRASP-Seq CBC Backbone

Pooled Library

#232068

Purpose

The CRASP-Seq backbone is designed to accommodate the cloning of diverse gRNA libraries. It includes a cell barcode (CBC), which enhances its functionality by enabling library preparation.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only
VWF-pHluorin-mRFP

Plasmid

#234367

Purpose

Von Willebrand factor fused in tandem to superecliptic pHluorin and inserted into pmRFP1-N1 vector from Addgene (Plasmid No: 54635)/Monitoring of WPB pH, Visualization of WPB exocytosis

Depositor

Volker Gerke

Article

Terglane et al PLoS One. 2022 Jun 29;17(6):e0270299. do

Insert

Von Willebrand factor (VWF Human)

Tags

pHluorin

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat PGK1 shRNA

Plasmid

#222869

Purpose

Expresses mTagBFP2 along with an shRNA against rat PGK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1 shRNA (Pgk1, Pgk1 Rat, Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB1

Plasmid

#200981

Purpose

Mammalian expression plasmid for myc-tagged EphB1

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB1 (EPHB1 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB6

Plasmid

#200984

Purpose

Mammalian expression plasmid for myc-tagged EphB6

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB6 (EPHB6 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB3-Fc

Plasmid

#200985

Purpose

Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB3 (EPHB3 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB3

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Expression

Mammalian

Mutation

A206K both

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pM423

Plasmid

#172408

Purpose

Expresses sfGFP (1-10) and MCP-GFP11

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

MCP, bipartite sfGFP

Use

CRISPR and Lentiviral

Tags

sfGFP {1-10}

Expression

Mammalian

Promoter

hPGK

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pM214

Plasmid

#173216

Purpose

Expresses MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU52

Plasmid

#167678

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET-NtLNtC60αC20C60β

Plasmid

#160874

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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