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Plasmid#69036Purposereporter with qacR 5 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationW61Y, E90Q, F102S, M116Q, Y119L, E120QPromoterpQacA and pTetAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only
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pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172208PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172205PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ConFoff-eGFP-W3SL
Plasmid#218751PurposeProvides NOT intersectional (Cre NOT Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL
Plasmid#218750PurposeProvides NOT intersectional (Flp NOT Cre) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ConFonvCon-eGFP-W3SL
Plasmid#218752PurposeProvides AND intersectional (Cre+Flp+vCre) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR
Plasmid#200636PurposeStrong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL
Plasmid#218753PurposeProvides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N1-FUS/TLS-FLAGC
Plasmid#60362PurposePlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.DepositorInsertFUS (FUS Human)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
UseTagsExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-GFP
Plasmid#58806PurposeAAV expression of ChrimsonR-GFP under the Syn promoterDepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationK176RPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CoChR-GFP
Plasmid#59070PurposeAAV-mediated expression of CoChR-GFP under the Syn promoterDepositorInsertCoChR-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
UseTagsExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Chronos-GFP
Plasmid#58805PurposeAAV expression of Chronos-GFP under the CaMKII promoterDepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2*
Plasmid#220246PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2* (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-rc[Chronos-GFP]
Plasmid#59056PurposeExpression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)DepositorInsertChronos-GFP
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-HA-Slc1a5 (mouse)
Plasmid#71458Purposeinducible expression fo Slc1a5 in mammalian cellsDepositorInsertSlc1a5 (Slc1a5 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationA85P, L125F and V384A mutations compared to GenBa…PromoterCMVAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only