We narrowed to 18,764 results for: Mut
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Plasmid#114182PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only
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pX68
Plasmid#46857PurposeOP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCEDepositorAvailable SinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M
Plasmid#114180PurposeORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN3 H196A-pcDNA3
Plasmid#22717DepositorInsertEgg laying Nine - 3 (EGLN3 Human)
TagsHA-tagExpressionMammalianMutationmutant cDNA: point mutation at codon 196 was ins…Available SinceDec. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-spTorA-GFP(C)
Plasmid#169041PurposeGFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.DepositorInsertH6-spTorA-GFP
Tags6xHis, TEV cleavage site, and spTorA (signal pept…ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/D614G
Plasmid#164570PurposeSARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)DepositorInsertSpike (S-RRAR-D614G variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationD614GAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3 H194L
Plasmid#122689PurposeExpresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
ExpressionMammalianMutationChanged Histidine 194 to LeucineAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 E179K
Plasmid#60541PurposeDonor vector for human MLK1 E179K mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 D294A
Plasmid#60542PurposeDonor vector for human MLK1 D294A mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM2CCtoSS
Plasmid#89154Purposerecombinant expression of PTBP1-RRM2 C250S,C251S in e.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
TagsIntein and chitin binding domainExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria1
Plasmid#124867PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ACVR1-TAAS-R206H
Plasmid#206184Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagMutationS190A, S192A, R206HAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hLMX1A-T2A-copGFP
Plasmid#170444PurposeLentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.DepositorInserthuman LMX1A
UseLentiviralPromoterEF1aAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Nkx2-1*/Neo
Plasmid#31272DepositorInsertNkx2-1* (Nkx2-1 Mouse)
UseRetroviralExpressionMammalianMutationA shNkx2-1 insensitive Nkx2-1 cDNA was created by…Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria3
Plasmid#124855PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-JAK1Gly590Arg
Plasmid#174573PurposeHuman FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)DepositorInsertJAK1Gly590Arg
TagsFLAGExpressionMammalianMutationGly590ArgPromoterCMVAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only