We narrowed to 150,487 results for: ins

1. pMJA104

   Plasmid

   #128520

   Purpose

   Expresses amyloid human Lysozyme in Pichia pastoris

   Depositor

   Marcos Alcocer

   Article

   Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

   Insert

   Artificial gene for human lysozyme I56T (LYZ Human)
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MBC1-PuroIII

   Plasmid

   #153419

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS ColIII alpha1

   Plasmid

   #19187

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Procollagen type III alpha 1 (COL3A1 Chicken)
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS GDF10

   Plasmid

   #19186

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Growth differentiation factor 10 precursor (GDF10 Chicken)
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pET30a-SARS-CoV2-ORF8

   Plasmid

   #165089

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pXPA-fapO-RgTALsyn

   Plasmid

   #100946

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   GAP (Constitutive)

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330 _HITI donor B6

   Plasmid

   #172847

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBacDual with LFn + PrgJ

    Plasmid

    #84869

    Purpose

    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    PrgJ
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV asyn S87E

    Plasmid

    #36057

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S87E

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

12. sgFFL

    Plasmid

    #59877

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HOXA9 (murine) FLAG pRC/CMV

    Plasmid

    #8514

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    FLAG epitope tag replaces T7 tag in the pET back…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. CBP-Bromo-HAT-CH3-sp65

    Plasmid

    #21026

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Mutation

    Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pCDB181

    Plasmid

    #80692

    Purpose

    for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

    Depositor

    David Baker

    Article

    Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

    Insert

    gEHEE_06
    

    Tags

    OsmY-10His-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330_rActb sgRNA / hSpCas9

    Plasmid

    #172833

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBC2-PuroIII

    Plasmid

    #153420

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid

    #172835

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LacI/109-pHG165c

    Plasmid

    #90058

    Purpose

    WT LacI polymorphism with T at position 109

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LacI/109
    

    Expression

    Bacterial

    Mutation

    109

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_rSyn1 sgRNA / hSpCas9

    Plasmid

    #172840

    Purpose

    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge