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Plasmid#142774PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDPF2 (DPF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E(D556A)
Plasmid#79564Purposeexpression of CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB63 - pL0_fLUC-I (CDS1)
Plasmid#123180PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plantsDepositorInsertLuciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
UseLuciferase; Part for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE-BI GFP10-RHOA/RBD-GFP11
Plasmid#182232PurposeInducible coexpression of GFP10-RHOA and RBD-GFP11 domainDepositorInsertsUseLentiviralTagsGFP10 (split-GFP tripartite) and GFP11 (M4)ExpressionMutationPromoterTRE bidirectionalAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyTagsExpressionYeastMutationPromoterGal1,10Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-INPP5E
Plasmid#79561Purposeexpression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-INPP5E(D556A)
Plasmid#79563Purposeexpression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS3 (CK2alpha, CK2beta)
Plasmid#27092DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianMutationPromoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)
Plasmid#204700PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of all TF-bidning domains and HAT in p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)
Plasmid#204699PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of all TF-bidning domains in p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS2 (CK2alpha', CK2beta)
Plasmid#27093DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianMutationPromoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10
Plasmid#180350Purposemammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10
Plasmid#180348Purposemammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10
Plasmid#180351Purposemammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGV7 (CK2alpha' K69M, CK2beta)
Plasmid#27095DepositorUseTetracycline-regulated expressionTagsHA tag on CK2alpha' and Myc on CK2betaExpressionMammalianMutationK69M on CK2alpha'Promoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10
Plasmid#180349Purposemammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10
Plasmid#180352Purposemammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2
Plasmid#180353Purposemammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationSEPT7 H279DPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only