We narrowed to 144,767 results for: ins

1. pMSCV-FKBP-IRF3

   Plasmid

   #221260

   Purpose

   Expression of IRF3 in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRF3 (Irf3 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-nef-CIAO2-Flp

   Plasmid

   #149296

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TAPBPL pmRFP-N2 (721)

   Plasmid

   #62000

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   TAPBPL (TAPBPL Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Use

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

   Plasmid

   #138334

   Purpose

   Expresses APOB-Linker-eGFP

   Depositor

   Mahmood Hussain

   Article

   Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

   Insert

   5'UTR-ApoB48-Linker-eGFP (APOB Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-nef-CIAO-Flp

   Plasmid

   #149294

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-CA-mDia1

   Plasmid

   #45583

   Depositor

   Klaus Hahn, Ronen Zaidel-Bar

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Insert mDia1 contains mutations V161D and N165D i…

   Promoter

   CMV

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. p6600 MSCV-IP N-HAonly JUN

    Plasmid

    #34898

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    JUN (JUN Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBabepuro3-p16Flag

    Plasmid

    #24934

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p16 (CDKN2A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSP64-hKCNQ1

    Plasmid

    #53048

    Purpose

    Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CRY2olig-mCh-Nck

    Plasmid

    #60034

    Purpose

    Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-NCK (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabepuro3-p18Flag

    Plasmid

    #24933

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    P18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

19. LADL Anchor

    Plasmid

    #127664

    Purpose

    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    dCas9-CIBN-2A-Puro
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 (D10A,H840A) mutant

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR P2R-P3-EGFP

    Plasmid

    #48349

    Purpose

    Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Aminoacid 40 is valine in our sequence, while it …

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge