We narrowed to 10,885 results for: pcas

Showing: 3041 - 3060 of 10885 results

Results per page:

Sort results by:

px551-mSncg-0.27kb-HA-SpCas9

Plasmid

#153208

Purpose

Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

Truncated mSncg (0.27kb)

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Tags

V5

Expression

Mammalian

Promoter

pCAGGS

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FIP200 (1-640)-TSF

Plasmid

#203545

Purpose

FIP200 NTD-TwinStrep-Flag mammalian expression

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-hChR2(H134R)-EYFP

Plasmid

#114367

Purpose

expresses channelrhodopsin 2 fused with EYFP

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

hChR2(H134R)

Tags

EYFP

Expression

Mammalian

Mutation

the Histidine at amino acid position 134 was chan…

Promoter

pCAG

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Expression

Mammalian

Mutation

amino acids 1–612

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-pac-T2A-iCre (v2)

Plasmid

#125821

Purpose

Puromycin resistance gene and iCre recombinase (version 2)

Depositor

Mario Capecchi

Insert

pac-T2A-iCre

Use

Cre/Lox

Expression

Mammalian

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

Plasmid

#148859

Purpose

Mammalian Expression of HsXRN1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsXRN1-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-evoSpCas9-NLS-6xHis

Plasmid

#207390

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-VSFP Butterfly 1.2

Plasmid

#47978

Purpose

Genetically encoded voltage indicator

Depositor

Thomas Knopfel

Article

Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

Insert

VSFP Butterfly 1.2

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Tet-on Advanced -BGH-PA

Plasmid

#114379

Purpose

Express reverse tetracycline inducible rtTA transcriptional activator

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Insert

rtTA and BGH-pA

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-pac-T2A-FLPo

Plasmid

#124835

Purpose

Puromycin resistance gene and FLP recombinase

Depositor

Mario Capecchi

Insert

pac-T2A-FLPo

Expression

Mammalian

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-sfGFP-iCre-ERT2

Plasmid

#125749

Purpose

sfGFP and iCreERT2 fused by unskippable linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

sfGFP-iCre-ERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cmv d0 dPspCas13b-GGS-NYTHDF1

Plasmid

#119855

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NRCH-ABE8.17-m+V106W

Plasmid

#198551

Purpose

Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-ABE8.17-m+V106W

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Synthetic, Human)

Tags

2XHA

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge