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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_NDG

Plasmid

#26820

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-NDGFP-3'UTR

Expression

Mammalian

Mutation

nuclear destabilized eGFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2188-PrARG8-GFP::URA3

Plasmid

#216512

Purpose

Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrARG8-yeGFP

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

ARG8

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB2197-PrGNP1-mK2-NatMX

Plasmid

#216513

Purpose

Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrGnp1-mKate2

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

GNP1

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV MCL1 Puro

Plasmid

#140746

Purpose

Expresses MCL1 in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

MCL1 (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS

Plasmid

#191367

Purpose

Overexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dMisCas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSMAL

Plasmid

#161785

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_d2eGFP

Plasmid

#26821

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-d2eGFP-3'UTR

Expression

Mammalian

Mutation

destabilized (PEST sequence) GFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191537

Purpose

Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIRESneo-EGFP-alpha Tubulin

Plasmid

#12298

Depositor

Patricia Wadsworth

Article

Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

Insert

EGFP-alpha Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-Syn1-mCherry

Plasmid

#222967

Purpose

Make lentivirus to express mCherry in syn1 expressing cells

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

mCherry

Use

Lentiviral

Promoter

synapsin I

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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