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pDONR223_STC2_p.R5W

Plasmid

#82744

Purpose

Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Mutation

R5W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Plasmid

#179105

Purpose

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Plasmid

#179116

Purpose

Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site for in-frame fusion

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.H1047R

Plasmid

#82824

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry vector

Mutation

H1047R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF2_WT

Plasmid

#82889

Purpose

Gateway Donor vector containing ATF2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ATF2 (ATF2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MDM2_WT

Plasmid

#82897

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GOPC_WT

Plasmid

#82924

Purpose

Gateway Donor vector containing GOPC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GOPC (GOPC Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MYC_WT

Plasmid

#82927

Purpose

Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

Myc (MYC Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAP1_WT_V5

Plasmid

#82994

Purpose

Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TRAP1 (TRAP1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.L858R

Plasmid

#82906

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-KLF2-T2A-mCHERRY

Plasmid

#224295

Purpose

Expresses mouse KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPP1_WT_V5

Plasmid

#82981

Purpose

Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SPP1 (SPP1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV uN2C GFP (Ctl)

Plasmid

#224355

Purpose

Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

Depositor

Nicolas Charlet-Berguerand

Article

Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

Insert

uN2C

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Codon-optimized for expression in human, so no pu…

Promoter

CMV + chimeric intyron (CAG)

Available Since

Oct. 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway entry vector

Mutation

E545K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SHC1_WT_V5

Plasmid

#82931

Purpose

Gateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SHC1 (SHC1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBR1_WT_V5

Plasmid

#82945

Purpose

Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CBR1 (CBR1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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