We narrowed to 8,346 results for: gnal

1. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #178087

   Purpose

   HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   mScarlet-I mTOR signaling indicator (TOSI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRS6E1b-luc(-732/-721) WT

   Plasmid

   #45383

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pTRE-tight2-Tmem98-FLAG

   Plasmid

   #124506

   Purpose

   Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)

   Depositor

   Renee van Amerongen

   Article

   van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

   Insert

   Tmem98 (Tmem98 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   tetO-CMV

   Available since

   May 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PM-CaNAR2

   Plasmid

   #64730

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal targeting sequence from Lyn…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOME0182

   Plasmid

   #178403

   Purpose

   Expresses KSHV K8.1 glycoprotein hyp tagged

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV K8.1
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-OMOR-eYFP

   Plasmid

   #66847

   Purpose

   Expresses Opto-MOR in a Cre dependent manner

   Depositor

   Michael Bruchas

   Article

   Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

   Insert

   Opto Mu opioid receptor
   

   Use

   AAV and Cre/Lox

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-CA-Src-GFP

   Plasmid

   #83469

   Purpose

   Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Src (SRC Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y527F (constitutively active)

   Promoter

   doxycycline-inducible promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-NIK

   Plasmid

   #111197

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   NIK (MAP3K14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mKate2-KDEL-pA

   Plasmid

   #109546

   Purpose

   Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2 CA-LRP6-GFP

    Plasmid

    #29682

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    LDL-receptor related protein 6 (LRP6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    extracellular region deleted

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBpuro-miRFP670-HRasCT

    Plasmid

    #176030

    Purpose

    Encoding membrane-targeted miRFP670

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Membrane-targeted miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. anti-Notch2_B6-pBIOCAM5

    Plasmid

    #39347

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV CAGG eGFP

    Plasmid

    #107707

    Purpose

    express eGFP in variety of cell types

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xDBE_RE::Renilla

    Plasmid

    #124535

    Purpose

    Foxo Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    FOXO Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-N2-PKCgamma

    Plasmid

    #21204

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKC gamma (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKC gamma (rat). (Lab plasmid WC 0013)

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. SEH1L in pRK5

    Plasmid

    #154216

    Purpose

    Untagged SEH1L cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    SEH1L (SEH1L Human)
    

    Use

    Tags

    NONE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GRK2 S670A

    Plasmid

    #45755

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 S670A (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S670A

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only