We narrowed to 6,999 results for: nsf

1. pET15b MOSPD2 [315-445]

   Plasmid

   #186474

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [315-445]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-SopB(172-554)

   Plasmid

   #183664

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 172-554

   Promoter

   CMV

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-SopB(211-554)

   Plasmid

   #183665

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 211-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAID1.2-EF1a-NmClover3-hsLem2

   Plasmid

   #179524

   Purpose

   Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mClover3-mAID

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR_P4-P1R:PER36modp

   Plasmid

   #128431

   Purpose

   Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

   Depositor

   George Haughn

   Article

   McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

   Insert

   PEROXISADE36 (AT3G50990 Mustard Weed)
   

   Use

   Gateway promoter entry clone

   Tags

   Expression

   Mutation

   Premature stop codons introduced into the Xero1 (…

   Promoter

   PEROXISADE36

   Available since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCri-H6-TEV-GRAB

   Plasmid

   #128498

   Purpose

   Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
   

   Use

   Tags

   His tag (6x)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCri-H6-TEV-GRAB-STREP

   Plasmid

   #128499

   Purpose

   Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
   

   Use

   Tags

   His tag (6x) and Strep tag (2x)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB61 - pL0_V2 (CDS1)

   Plasmid

   #123183

   Purpose

   Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
   

   Use

   Part for plant expression

   Tags

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/ZMYM2-FGFR1

    Plasmid

    #167486

    Purpose

    Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

    Insert

    ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-PRMT3(E338Q)

    Plasmid

    #164696

    Purpose

    Expression of PRMT3(E338Q) with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

    Insert

    PRMT3 (PRMT3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E338Q

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVE-hTGFB3-IRES-Red

    Plasmid

    #52580

    Purpose

    2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    human transforming growth factor-beta3 (TGFB3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/BCR-FGFR1

    Plasmid

    #167490

    Purpose

    Mammalian expression vector for the BCR-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Demiroglu et al Blood. 2001 Dec 15;98(13):3778-83. doi:

    Insert

    BCR-FGFR1 (BCR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSMT-MjtRNA-GFP(47TAG)

    Plasmid

    #193251

    Purpose

    Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

    Depositor

    Jessica Seeliger, Peter Schultz

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Inserts

    GFP(47TAG)
    MjtRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon for Tyr47 replaced with amber stop codon TA…

    Promoter

    hsp60 and metU

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Epac-S-H30

    Plasmid

    #170337

    Purpose

    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

    Insert

    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO.neo_shGFP

    Plasmid

    #110470

    Purpose

    Control, silence GFP gene, doxycycline inducible, neomycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO (RNA PolIII)

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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