We narrowed to 17,427 results for: emb

1. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

   Plasmid

   #170376

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHA850 - osm-10p::FynY531F

   Plasmid

   #139207

   Purpose

   Express activated Fyn kinase in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Expression

   Worm

   Mutation

   Y531F

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHA851 - osm-10p::Fynempty

   Plasmid

   #139208

   Purpose

   Control vector for Fyn kinase expression in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUS_LC_withR

   Plasmid

   #139128

   Purpose

   express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Mutation

   add arginines

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hnRNPA2_LC_nocharge_wR

   Plasmid

   #139119

   Purpose

   express hnRNPA2 LC with no charged residues with R added back

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Expression

   Bacterial

   Mutation

   remove all K, most D

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUS_LC_charge

   Plasmid

   #139126

   Purpose

   express FUS LC with an hnRNPA2 LC-like charge patterning

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Mutation

   Add R, K, D to make FUS LC have similar charge pa…

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Miro1 (1-177 fused to 562-618)

   Plasmid

   #127627

   Purpose

   First GTPase domain of human Miro1 fused to C-terminus

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   1-177 fused to 562-618

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Oct4deltaPE-53BP1mCherry

   Plasmid

   #141122

   Purpose

   Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

   Depositor

   John Schimenti

   Article

   Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

   Insert

   Fragment of human 53BP1 (TP53BP1 Human)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NKP91 GFP (1034)

    Plasmid

    #62023

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    Tmem41A (TMEM41A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAV10.F3.loxP

    Plasmid

    #63200

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV10.HO5.loxP

    Plasmid

    #63206

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-UBC-XRI-HA

    Plasmid

    #178056

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-HA
    

    Use

    AAV

    Tags

    HA-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TO-NOD2-EGFP

    Plasmid

    #131207

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD2 (NOD2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP VAMP7 (1-220)

    Plasmid

    #42316

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 (1-220) (VAMP7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRPML1-G-GECO1.2-ERES

    Plasmid

    #207144

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Mcoln1 (MCOLN1 Human)
    

    Tags

    ER-Export Signal (ERES) and G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-IRF9

    Plasmid

    #71452

    Purpose

    Lentiviral expression of IRF9

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. W118-1_hZBTB7B

    Plasmid

    #180380

    Purpose

    lentiviral transduction of human ZBTB7B gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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