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Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb

Plasmid

#192436

Purpose

Encodes mCherry-tagged wild-type Rheb.

Depositor

Jin Zhang

Publication

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a-3xV5

Plasmid

#182554

Purpose

Expresses Nav1.6 tagged with 3xV5

Depositor

Zhe J. Liu

Publication

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Tags

3xV5

Expression

Mammalian

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Publication

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFAR5

Plasmid

#149478

Purpose

Broad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

AraC-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWLxR5

Plasmid

#149490

Purpose

Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-Alus-Apobec3G

Plasmid

#92348

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Publication

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pssGFP-hCRT

Plasmid

#80022

Purpose

Mammalian expression of signal peptide-EGFP-humanCRT

Depositor

Chinten James Lim

Publication

Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

Insert

CRT (CALR Human)

Tags

EGFP and signal peptide

Expression

Mammalian

Mutation

signal peptide 17aa added before GFP-CRT, will ta…

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Expression

Worm

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBadcrtEBI_13

Plasmid

#63811

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-TRP1

Plasmid

#26877

Depositor

Peter Kaiser

Publication

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6WT-HA

Plasmid

#239762

Purpose

Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Diffusive 67kDa

Plasmid

#201338

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-6PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Publication

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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MIGR1-CD5

Plasmid

#159329

Purpose

Over-expression of mouse CD5

Depositor

Nevil Singh

Publication

Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

CD5 (Cd5 Mouse)

Use

Retroviral

Promoter

LTR

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-Halo-10-PFN1

Plasmid

#204222

Purpose

Expression of Halo-Profilin-1 in tissue culture cells

Depositor

Jessica Henty-Ridilla

Publication

Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

Insert

PFN1 (PFN1 Human)

Tags

Halo, TEV site, SGLRSRAQAS linker

Expression

Mammalian

Promoter

CMV

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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