We narrowed to 5,908 results for: pcDNA 3.1

1. pcDNA3-HA-REDD1 N207-225 (#439)

   Plasmid

   #99938

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delAA207-225insNAAIRS linker

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-REDD1 N109-119 (#432)

   Plasmid

   #99933

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delAA109-119insNAAIRS linker

   Promoter

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-HA/Bmal L95E/L150E/W427A

   Plasmid

   #47352

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E/L150E/W427A

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3-HA/Bmal F423R/V435R/W427R

   Plasmid

   #47351

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   F423R/V435R/W427R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-myc-his-BACH1 K52R

   Plasmid

   #17643

   Depositor

   Ronny Drapkin

   Article

   Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

   Insert

   BACH1 (BRIP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   The lysine residue at position 52 was changed to …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA Flag PGC1 T177A S538A

   Plasmid

   #18093

   Depositor

   Bruce Spiegelman

   Article

   Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T177A S538A

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-flag-hKDM4A(H188A)-polyA

   Plasmid

   #101052

   Purpose

   in vitro transcription (T7 promoter)

   Depositor

   Yi Zhang

   Article

   Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

   Insert

   KDM4A (KDM4A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

   Plasmid

   #141184

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Has Service

   Cloning Grade DNA

   Insert

   S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
   

   Use

   Tags

   Signal peptide from influenza HA and Superfolder …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Use

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-myc-his-BACH1 P47A

    Plasmid

    #17644

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    To generate the tumor-associated mutant, the prol…

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-GFP-IMP2-2_antisense-control

    Plasmid

    #42174

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-EGFR*-CUbo-His6

    Plasmid

    #212819

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    CUbo-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

    Plasmid

    #212822

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    CUbo(K63R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2-Cx43-M100/125L-siResist

    Plasmid

    #49855

    Purpose

    Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100 and Methionine 125 mutated to Leuci…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA flag PGC1 (180-797)

    Plasmid

    #8943

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-R536C-WLS-HA-IRES-GFP

    Plasmid

    #178065

    Purpose

    Expression of WLS-HA protein (R536C mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    R536C

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Y478C-WLS-HA-IRES-GFP

    Plasmid

    #178063

    Purpose

    Expression of WLS-HA protein (Y478C mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    Y478C

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge