We narrowed to 9,990 results for: SUB;

1. pMIR-Dsred-IRES-His Keap

   Plasmid

   #62457

   Purpose

   expresses his tagged human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   HisTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT-R16

   Plasmid

   #31827

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   mini Tol2 ITR
   

   Use

   Cre/Lox; Gene trap

   Tags

   mRFP

   Mutation

   mRFP lacks start codon

   Promoter

   beta Actin

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. optoPKAW196R

   Plasmid

   #104548

   Purpose

   Expresses Cry2-mCherry-PKA W196R in mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Cry2-mCherry-PKA W196R
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   PKA W196R

   Promoter

   CMV

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet RagA-RagC(S75N)

   Plasmid

   #99645

   Purpose

   Bacterial expression of RagA-RagC(S75N)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S75N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSSK p85 alpha (delta SH3)

   Plasmid

   #13430

   Depositor

   Ronald Kahn

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

   Insert

   p85 alpha (delta SH3) (PIK3R1 Human)
   

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   SH3 domain replaced by flag tag.

   Available Since

   Nov. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pGBW-m4046940

   Plasmid

   #149261

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJOE5751.1

   Plasmid

   #135083

   Purpose

   pBR322-based L-rhamnose-inducible vector.

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

   Insert

   P-rhaP-BAD-His tag-eGFP
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBI-CMV1-mir421-CMV2-mir1

   Plasmid

   #164673

   Purpose

   Express miR-421 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-421 (MIR421 Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS DKK3

   Plasmid

   #19201

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Dickkopf homolog 3 (DKK3 Chicken)
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGMC00014

    Plasmid

    #172526

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Non-targeting guide
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-MRPP1_native+

    Plasmid

    #67865

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form)

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP1 (TRMT10C Human)
    

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-GSX2-tTA-BleoR

    Plasmid

    #161748

    Purpose

    Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    Advanced tTA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-HsSENP5

    Plasmid

    #71468

    Purpose

    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pICH-chl-roGFP2-GPXC71S

    Plasmid

    #191703

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-redox(ro)GFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-SYN-integrin alpha9-V5

    Plasmid

    #203729

    Purpose

    AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    ITGA9 (ITGA9 Human)
    

    Use

    AAV

    Tags

    V5-tag

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSSK p85 alpha (delta BH)

    Plasmid

    #13431

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta BH) (PIK3R1 Human)
    

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    BH domain replaced by flag tag.

    Available Since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pBI-CMV1-mir181b-1-CMV2-mir1

    Plasmid

    #164670

    Purpose

    Express miR-181b-1 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-181b-1 (MIR181B1 Human)
    miR-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046925

    Plasmid

    #145694

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pyc128m

    Plasmid

    #158778

    Purpose

    soluble GCaMP expression

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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