We narrowed to 166,037 results for: addgene

1. pLKO.1 RagB shRNA #1

   Plasmid

   #26627

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagB shRNA (RRAGB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pX330-NEAT1m_v1

   Plasmid

   #97083

   Purpose

   Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1m_v1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMVTCRb_candidate10

   Plasmid

   #165008

   Purpose

   Expression of candidate TCR #10 TCRbeta cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   TRB (TRB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLIK 3XFLAG-EGFP-NLS hygro

   Plasmid

   #87781

   Purpose

   Inducible expression of EGFP with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RIB VP64 R1

   Plasmid

   #161493

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R1 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX335-NEAT1_IS_v1

   Plasmid

   #97085

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CBh-DIO-CB2-EGFP

   Plasmid

   #91946

   Purpose

   Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Inserts

   EGFP
   type-2 cannabinoid receptor
   

   Use

   AAV

   Tags

   FLAG and None

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX335-NEAT1_IS_v2

   Plasmid

   #97086

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v2
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRG645_lexO-AscI_LexA-TF_LEU2MX

    Plasmid

    #154813

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AIRE_pCSdest

    Plasmid

    #53891

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    AIRE
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pLJM1 Flag Raptor HRas25

    Plasmid

    #26637

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor-HRas25 (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pSL-1

    Plasmid

    #180422

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Red Chromprotein gene
    

    Expression

    Bacterial

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIPlac204TKC-E2-Crimson-HDEL

    Plasmid

    #38771

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Tags

    Linker peptide and yeast ER retention signal sequ…

    Expression

    Yeast

    Promoter

    TPI1

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICH-roGFP2-GPXC71S

    Plasmid

    #191698

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-GPXC71S
    

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe puro MTH1

    Plasmid

    #21295

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    MTH1 (NUDT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pET22b-GFP-CPDSalI

    Plasmid

    #38257

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    eGFP
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pEGFPN3 (Clonetech)

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. 5xHRE-MinTK-CRE

    Plasmid

    #141145

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    5xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSADdeltaG-ChR2-mCherry

    Plasmid

    #32646

    Purpose

    Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    ChR2
    

    Use

    Rabies virus

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge