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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,945 results for: lif

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Showing: 6241 - 6280 of 7945 results

  1. J-GALTA-3

    Plasmid
    #166523
    Purpose
    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166523-plasmid-map-sequence-id-329136

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  2. A-PRMT3-3

    Plasmid
    #166514
    Purpose
    scFv of a human scaffold targeting Protein arginine methyltransferase 3 isoform 1. Antigen coverage aa 211-531 of 531.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166514-plasmid-map-sequence-id-329056

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  3. Q-PHIPA-23

    Plasmid
    #166547
    Purpose
    scFv of a human scaffold targeting PH-interacting protein. Antigen coverage aa 1302 - 1434 of 1821, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166547-plasmid-map-sequence-id-328319

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  4. C-BRD1-3

    Plasmid
    #166517
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 1. Antigen coverage aa 925-1049 of 1058, PWWP domain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166517-plasmid-map-sequence-id-328311

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  5. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

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  6. O-AASSA-25

    Plasmid
    #166537
    Purpose
    scFv of a human scaffold targeting Aminoadipate-semialdehyde synthase. Antigen coverage aa 455-926 of 926
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166537-plasmid-map-sequence-id-328275

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  7. Plekhm1 C279 pGEX 4T2-GST

    Plasmid
    #73835
    Purpose
    Express human Plekhm1 (C279 to end domain) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned last 279 aa from human Plek…
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. Plekhm1 -PH1/2 pGEX 4T2-GST

    Plasmid
    #73831
    Purpose
    Express human Plekhm1 (PH1 and PH2 domains) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned Ph1 and Ph2 domains of human…
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. cacna2d1 pcDNA3

    Plasmid
    #206103
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206103-plasmid-map-sequence-id-415385

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  14. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

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  15. pCMV-SUN1 mScarlet

    Plasmid
    #187683
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Insert
    SUN1 (Sun1 Mouse)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187683-plasmid-map-sequence-id-371441

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  16. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  17. pMT-IMD-V5-His

    Plasmid
    #63748
    Purpose
    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    immune deficiency (imd Fly)
    Tags
    V5-6XHis
    Expression
    Insect
    Mutation
    *see comment below
    Promoter
    pMT
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63748-plasmid-map-sequence-id-108846

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  20. Q-IL6-9

    Plasmid
    #166552
    Purpose
    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166552-plasmid-map-sequence-id-328267

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  24. MAP3K6-TAP

    Plasmid
    #69727
    Purpose
    Express tandem tagged MAP3K6 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 2 (MAP3K6 Human)
    Tags
    HA-V5 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    69727-plasmid-map-sequence-id-128182

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  27. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166564-plasmid-map-sequence-id-327819

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  28. Q-IL18-6

    Plasmid
    #166554
    Purpose
    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166554-plasmid-map-sequence-id-329244

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  29. pMT-TRAF6-V5-His

    Plasmid
    #63752
    Purpose
    Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TRAF6 (Traf6 Fly)
    Tags
    V5-His
    Expression
    Insect
    Promoter
    pMT
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63752-plasmid-map-sequence-id-106437

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  30. pET11b-MIF Y99F

    Plasmid
    #149439
    Purpose
    Expression of MIF Y99F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Expression
    Bacterial
    Mutation
    Y99F
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149439-plasmid-map-sequence-id-311578

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  32. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  33. A-BRDA4-3

    Plasmid
    #166513
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166513-plasmid-map-sequence-id-328227

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  35. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

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  38. pGEX4T-1-Nix

    Plasmid
    #100759
    Purpose
    Bacterial expression of GST fused Nix protein
    Depositor
    Joseph Gordon
    Insert
    GST-Nix (BNIP3L Human)
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100759-plasmid-map-sequence-id-193048

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  39. Z-FARSB-5

    Plasmid
    #166556
    Purpose
    scFv of a human scaffold targeting phenylalanyl-tRNA synthetase, beta subunit. Antigen coverage aa 1-589 of 589, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166556-plasmid-map-sequence-id-329264

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Showing: 6241 - 6280 of 7945 results