We narrowed to 59,421 results for: cloning

1. PP-CDPK4 (3IGO)

   Plasmid

   #25192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   cgd3_920:T71-E538
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains amino acids T71-E538

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. PP-CDPK6 (3IS5)

   Plasmid

   #25194

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   38.m00014:T112-A378
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids T112-A378

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. PP-CPUF2

   Plasmid

   #25195

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PFE0790c (PFE0790c Plasmodium falciparum)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. PP-CDPK3 (3K21)

   Plasmid

   #25270

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PFC0420w:I388-L560
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids I388-L560

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. PP-MECPS

   Plasmid

   #25118

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pv003920:C34523 (PVX_003920 Plasmodium vivax)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. PP-CDPK8

   Plasmid

   #25132

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   cgd7_1840:C29742 (cgd7_1840 Cryptosporidium parvum)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. PP-RNR

   Plasmid

   #25135

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PY03671:C21955 (PY03671 Plasmodium yoelii)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

   Plasmid

   #229826

   Purpose

   Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I
   

   Use

   AAV

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSI-218

   Plasmid

   #197139

   Purpose

   All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SpCas9-PmCDA1-UGI

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-CS-060

    Plasmid

    #197133

    Purpose

    CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    MCP(N55K)

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hEF1a, U6

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV GFP DEST (736-1)

    Plasmid

    #19732

    Purpose

    3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Gateway destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p5E-EF1a/b-actin

    Plasmid

    #82583

    Purpose

    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVenusNfN

    Plasmid

    #128564

    Purpose

    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

    Depositor

    Oliver Vugrek

    Article

    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

    Insert

    Venus
    

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVenusCfC

    Plasmid

    #128567

    Purpose

    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

    Depositor

    Oliver Vugrek

    Article

    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

    Insert

    Venus
    

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVenusCfN

    Plasmid

    #128566

    Purpose

    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

    Depositor

    Oliver Vugrek

    Article

    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

    Insert

    Venus
    

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    human OCT4

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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