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We narrowed to 7,695 results for: Pol

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Showing: 3141 - 3160 of 7695 results

  5. pFastBacDual with LFn + FlaAAA

    Plasmid
    #84867
    Purpose
    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Publication
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    FlaAAA
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    Polyhedrin
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84867-plasmid-map-sequence-id-162897

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  6. 5E26 (PANK2)

    Plasmid
    #79516
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK2 (PANK2 Human)
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Promoter
    polyhedrin
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79516-plasmid-map-sequence-id-150092

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  9. Col2.3deltaTK

    Plasmid
    #114273
    Purpose
    Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.
    Depositor
    David Rowe
    Publication
    Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Col2.3 promoter + deltaTK + BGH polyA
    Expression
    Mammalian
    Promoter
    2.3 kb rat type 1 collagen
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114273-plasmid-map-sequence-id-220087

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  15. huntingtin_FL_NbioCflag

    Plasmid
    #194767
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194767-plasmid-map-sequence-id-402615

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  16. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid
    #193713
    Purpose
    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-L-mCherry-CAAX
    Tags
    2xFKBP-mCherry
    Expression
    Mammalian
    Mutation
    mCherry: Met1 Deleted
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193713-plasmid-map-sequence-id-383743

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  18. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162681-plasmid-map-sequence-id-321444

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  19. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162682-plasmid-map-sequence-id-320836

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Showing: 3141 - 3160 of 7695 results