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pCOM4

Plasmid

#49798

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Publication

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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ABE-NW1

Plasmid

#240615

Purpose

Adenine base editor with narrowed editing window

Depositor

Tingting Jiang

Publication

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)

Use

CRISPR

Expression

Mammalian

Mutation

TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pIDK-HDAC1fl

Plasmid

#109158

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Publication

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)

Expression

Insect

Promoter

p10

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHPK1212

Plasmid

#8834

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

tobacco vein mottling virus protease, catalytic domain

Tags

MBP

Expression

Bacterial

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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Reporter-1

Plasmid

#180218

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Publication

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HHM-iATPSnFR1.1

Plasmid

#102547

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.1

Expression

Bacterial

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRG205MX

Plasmid

#64535

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGGZ001

Plasmid

#48868

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

Plasmid

#157744

Purpose

knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Publication

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTALETF_v2 (NG)

Plasmid

#32186

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NG for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTALETF_v2 (HD)

Plasmid

#32188

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, HD for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNBU2_erm_P1T_DP-GH023

Plasmid

#90317

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Publication

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Publication

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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EF1α-mNeonGreen-Z1

Plasmid

#135613

Purpose

mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

Depositor

Jordan Green

Publication

Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a-HTLV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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