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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,138 results for: eng

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Showing: 3141 - 3160 of 14138 results

  2. pLSLZ.D.R

    Plasmid
    #51510
    Purpose
    Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS
    Promoter
    2x35S
    Available Since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51510-plasmid-map-sequence-id-78634

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  4. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  5. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  6. pCAG-N2cP

    Plasmid
    #100808
    Purpose
    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain phosphoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100808-plasmid-map-sequence-id-189510

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  8. pcDNA3-sACE2v2.4-8his

    Plasmid
    #149664
    Purpose
    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149664-plasmid-map-sequence-id-298418

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  9. pRSF-Ivy-his-hLYZ

    Plasmid
    #83920
    Purpose
    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
    Depositor
    Karl Griswold
    Article
    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
    Inserts
    Ivy
    lysozyme (LYZ Human)
    Tags
    Ivy protein has C-terminal hisx6 tag
    Expression
    Bacterial
    Mutation
    Ivy leader sequence deleted and replaced.
    Promoter
    T7 and t7
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83920-plasmid-map-sequence-id-160356

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  10. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid
    #127239
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127239-plasmid-map-sequence-id-247086

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  15. pV1200

    Plasmid
    #111429
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111429-plasmid-map-sequence-id-212464

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Showing: 3141 - 3160 of 14138 results