We narrowed to 7,548 results for: ski

1. piGECI-N1

   Plasmid

   #160421

   Purpose

   Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-Aka-P2A-GFP

   Plasmid

   #120867

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insert Akaluc and a P2A site

   Promoter

   CAG Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169317

   Purpose

   Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   IRES-dTomato

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC-NRP1

   Plasmid

   #158384

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   NRP1 (NRP1 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET51b-His-TEV-CLIP-tag

   Plasmid

   #167274

   Purpose

   Overexpression of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY14

   Plasmid

   #191059

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5(orf) (acr-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA5/FRT/TO_TOMM20_HaloTag7

   Plasmid

   #169330

   Purpose

   Expression of TOMM20-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag7 (TOMM20 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-NR1a

   Plasmid

   #17926

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET151-GyrB

   Plasmid

   #216233

   Purpose

   S. aureus USA300 GyrB Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ10333

    Plasmid

    #183955

    Purpose

    CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    Nuclease-dead Lb Cas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIB81-pmGFP

    Plasmid

    #75366

    Purpose

    Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    prenylated EGFP

    Expression

    Mammalian

    Mutation

    residues 1-81 of CIB1

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSUPER-retro-puro-tetO-CTR1-shRNA

    Plasmid

    #53180

    Purpose

    Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. SIN40C.SFFV.Luciferase.IRES.dTomato

    Plasmid

    #169307

    Purpose

    Constitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-dTomato

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Paf1

    Plasmid

    #11059

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    Paf1 (PAF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAC3-EGFR

    Plasmid

    #222610

    Purpose

    Expression of MAC3-tagged EGFR

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

    Plasmid

    #172516

    Purpose

    Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    C-term Nes HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172503

    Purpose

    Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

    Plasmid

    #193823

    Purpose

    CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    [4xCox8]-ChemoG-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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