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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,211 results for: CIT

Showing: 3161 - 3180 of 7211 results

  3. HEpl016

    Plasmid
    #179991
    Purpose
    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    SiCas12i
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179991-plasmid-map-sequence-id-358514

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  5. pPodA11

    Plasmid
    #162843
    Purpose
    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance
    Depositor
    Dianne Newman
    Article
    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11
    Insert
    Protein Designed Pyocyanin demethylase
    Use
    Tags
    TEV-cleavable 6x-His tag
    Expression
    Bacterial
    Mutation
    A53N, I73T, A87V, M99V, A129T from WT PodA
    Promoter
    T7
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162843-plasmid-map-sequence-id-343491

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  6. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

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  8. pGG438

    Plasmid
    #165486
    Purpose
    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, S1136R, D1332K, and R1333G mutations in S…
    Promoter
    CMV and T7
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165486-plasmid-map-sequence-id-330963

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  10. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid
    #124300
    Purpose
    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    SENP8 (SENP8 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124300-plasmid-map-sequence-id-242478

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Showing: 3161 - 3180 of 7211 results