We narrowed to 3,808 results for: MAK;

1. pMCSGC-GB1

   Plasmid

   #186786

   Purpose

   E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p221z-dCAS9p-t35s

   Plasmid

   #118387

   Purpose

   Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   dCAS9p
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALED_Right-stuffer-1397N

   Plasmid

   #187172

   Purpose

   original vector to make all constructs

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   Right-stuffer-1397N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pERB221

   Plasmid

   #61502

   Purpose

   const. CenpB-GFP-Halo + mCherry-DHFR

   Depositor

   Michael Lampson, David Chenoweth

   Article

   Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

   Inserts

   CenpB-GFP-Halo
   mCherry-DHFR
   

   Use

   Tags

   HaloTag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pERB217

   Plasmid

   #61500

   Purpose

   Const. Halotag-GFP-Mito + mCherry-DHFR

   Depositor

   Michael Lampson, David Chenoweth

   Article

   Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

   Inserts

   HaloTag-GFP-Mito
   mCherry-DHFR
   

   Use

   Tags

   HaloTag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLC242-GFP-Sestrin2

   Plasmid

   #100519

   Purpose

   Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDZ585 pKAN 2x-mCherry

   Plasmid

   #72236

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   2x-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-Lifeact-mEos2-NS

   Plasmid

   #115549

   Purpose

   Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Lifeact-mEos2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p221z-AtMIR390a

   Plasmid

   #118388

   Purpose

   Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   AtMIR390a backbone
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pERB219

    Plasmid

    #61501

    Purpose

    Const. Halotag-GFP-PACT + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    HaloTag-GFP-PACT
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H2B-mGarnet

    Plasmid

    #104311

    Purpose

    codes for a fusion of fluorescent protein mGarnet and human histon 2B (H2B), makes the H2B visible in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

    Insert

    H2B-mGarnet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330-USP13

    Plasmid

    #61812

    Purpose

    to make USP13 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pJC225

    Plasmid

    #176883

    Purpose

    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-6NBC-GFP-PGK-Puro-Barcode1

    Plasmid

    #83678

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTAPmyc-T2A-tagRFP

    Plasmid

    #140278

    Purpose

    Backbone for making a mammalian expression vector of the protein of interest

    Depositor

    Hirohide Saito

    Article

    Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

    Insert

    Empty backbone
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TALED_Left-stuffer-8e-E1347A

    Plasmid

    #187163

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Left-stuffer-8e-E1347A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TALED_Left-stuffer-1397C-UGI

    Plasmid

    #187168

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Left-stuffer-1397C-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

    Plasmid

    #205275

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    E498R

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

    Plasmid

    #205276

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    T513K

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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