We narrowed to 8,728 results for: Set

1. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Rock1-GBD (840-110 aa) in pEGFPN1

   Plasmid

   #187279

   Purpose

   Express EGFP-Rock1 GTPase-binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Rock1 (Rock1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GBD (84-110aa)

   Promoter

   CMV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH666

   Plasmid

   #169610

   Purpose

   Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH664

   Plasmid

   #169608

   Purpose

   Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH663

   Plasmid

   #169607

   Purpose

   Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

   Plasmid

   #178096

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + MTOR nick exon-44 sgRNAs
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

   Plasmid

   #178099

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + MTOR nick intron-45 sgRNAs
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

   Plasmid

   #178102

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

   Plasmid

   #178103

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178105

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid

    #178106

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick intron-45 sgRNAs
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid

    #178108

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA

    Plasmid

    #178109

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

    Plasmid

    #173209

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid

    #173211

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid

    #173212

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-TAC-OKMS-KRAB-dCas9

    Plasmid

    #120356

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid

    #120357

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid

    #163927

    Purpose

    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only