We narrowed to 22,967 results for: his

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 T239D

Plasmid

#22220

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn_NES-His-rsCaMPARI-mRuby3

Plasmid

#120805

Purpose

AAV plasmid for expressing rsCaMPARI in neurons, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

AAV

Tags

NES-His and mRuby3

Promoter

hsyn (synapsin-1)

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pET-28a-His6-SUMO-VAMP3

Plasmid

#135552

Purpose

Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP3

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pUb-HisUb-Ubc9-ChE1 v4.5

Plasmid

#185756

Purpose

Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Inserts

His-Ub
Ubc9
ChE1 v4.5

Tags

His6

Mutation

K14R

Promoter

T7

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p6268 pDEST-59-His-Trx-HPV16E6

Plasmid

#24126

Depositor

Peter Howley

Insert

HPV16 E6 (E6, E6 HPV)

Tags

His and Trx

Expression

Bacterial and Mammalian

Mutation

none

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-eGFP-dspB(E184Q)-6xHis

Plasmid

#175783

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag and eGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-dHer1cys

Plasmid

#73218

Purpose

Bacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)

Depositor

Marcel Bruchez

Article

Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

Insert

10XHis-GST-HRV-dHer1cys

Tags

10XHis-GST-HRV and The dimer of the Her1 Affibody…

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-Xyl-DocI

Plasmid

#58711

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Xyl-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Threonin 120 to Cysteine in Xylanase Doma…

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3

Plasmid

#198141

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCI_E01_mini-mAgrin-Myc-His-WPRE__LG3

Plasmid

#198140

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast PhLP1-myc-6xHis

Plasmid

#218178

Purpose

Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Use

Lentiviral

Tags

6x His and Myc

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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