We narrowed to 3,695 results for: D10

1. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD1025 EF1alpha TetON3G in TU6

   Plasmid

   #139262

   Purpose

   Expresses TetON3G under hEF1alpha in TUPV6

   Depositor

   Joshua Leonard

   Insert

   TetON3G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1alpha

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD1026 EF1alpha TetON3G in TU2

   Plasmid

   #139258

   Purpose

   Expresses TetON3G under hEF1alpha in TUPV2

   Depositor

   Joshua Leonard

   Insert

   TetON3G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1alpha

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD107 VP16-ZF43(RARR)

   Plasmid

   #138812

   Purpose

   Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF43(RARR)
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R15A

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hRad51(G151D)–Cas9(D10A)

   Plasmid

   #125565

   Purpose

   Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(G151D)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-GW1D10(aa566-1343)

   Plasmid

   #21517

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain aa566-1343

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

7. hRad51(F86E)–Cas9(D10A)

   Plasmid

   #125564

   Purpose

   Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(F86E)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GST-GW1D10(aa566-1343)

   Plasmid

   #21516

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contain aa566-1343

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Top7 D9T D10V N14T

   Plasmid

   #12473

   Depositor

   David Baker

   Insert

   Top7 D9T D10V N14T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D9T D10V N14T

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-nEF-SpCas9D10A nickase

    Plasmid

    #216736

    Purpose

    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF-1-alpha core

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid

    #199254

    Purpose

    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KARA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A R1060A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid

    #139257

    Purpose

    TRE3GV promoter and DsRedExpress2 in TUPV5

    Depositor

    Joshua Leonard

    Insert

    DsRedExpress2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPD109 VP16-ZF43(RAAA)

    Plasmid

    #138814

    Purpose

    Constitutive expression of VP16-ZF43(RAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(RAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD108 VP16-ZF43(RAAR)

    Plasmid

    #138813

    Purpose

    Constitutive expression of VP16-ZF43(RAAR) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(RAAR)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A, R43A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2CT-His-MBP-Lbu_C2c2_D1078A

    Plasmid

    #91860

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) D1078A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    D1078A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge