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Plasmid#183809Purposehis plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.DepositorInsertAAV transgene TetO promoter-DIO/flex-GCaMP6
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6-210
Plasmid#91778PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP6-210
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralTagsExpressionMammalianMutationPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SomaGCaMP7
Plasmid#182941PurposeCre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2386-Tier1-PhCMV-GCaMP6s
Plasmid#169519PurposeTier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).DepositorInsertPCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8s
Plasmid#176761PurposeAAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8s
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-GCaMP7s
Plasmid#172385PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + GCaMP7s
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP6f-P2A-PAmCherry
Plasmid#133412PurposeFluorescent reporter for calcium imaging and photoactivation.DepositorInsertGCaMP6f-P2A-PAmCherry
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-GCaMP6s
Plasmid#166606PurposeEncodes Cre-dependent GCaMP6s under control of the TREDepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-mDlx-jGCaMP8m-WPRE
Plasmid#176754PurposejGCaMP8m expression in GABA-ergic interneurons under the control of mDlx enhancerDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromotermDlxAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
QUAS-jGCaMP7s-T2A-tdTomato
Plasmid#200010PurposeQUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2ADepositorInsert3xP3-ECFP-jGCaMP7s-td-Tomato
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8f
Plasmid#169258PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.DepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertSoma-jGCaMP8f
UseAAVTags6xHis-tagExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f
Plasmid#72304PurposeCombines a gCaMP-based calcium indicator and an Arch-based voltage indicatorDepositorInsertCaViar
UseLentiviralTagsTSExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE
Plasmid#176752PurposeAAV-mediated expression of GCaMP8s under the CamKIIa promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP5g-P2A-PAmCherry
Plasmid#133411PurposeFluorescent reporter for calcium imaging and photoactivation.DepositorInsertGCaMP5g-P2A-PAmCherry
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Soma-jGCaMP8f
Plasmid#182943PurposeCell body-targeted jGCaMP8f, under synapsin promoter.DepositorInsertSoma-jGCaMP8f
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8s-WPRE
Plasmid#176758PurposeAAV-mediated expression of GCaMP8s under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only