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pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]

Plasmid

#229388

Purpose

XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropout

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Type

Empty backbone

Expression

Bacterial

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LCAPCW

Plasmid

#205646

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

hCMV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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LMAPPW

Plasmid

#205657

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L1-Donor-Esp3I

Plasmid

#197842

Purpose

Donor vector to restore L1 destination via Esp3I

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCC12

Plasmid

#198767

Purpose

Drives specific expression in the ADF neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrh-142-GAL4-SK(DBD)-VP64

Expression

Worm

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP026

Plasmid

#198800

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrb-6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-Ura-Cen-lvl1

Plasmid

#176182

Purpose

preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ssRNb-GFP

Plasmid

#128791

Purpose

Mammalian expression

Depositor

Colin Taylor

Publication

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssRNb-GFP

Expression

Mammalian

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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RNb-zdk1

Plasmid

#128781

Purpose

Mammalian expression

Depositor

Colin Taylor

Publication

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-zdk1

Expression

Mammalian

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD-Cyc1t (17-21)

Plasmid

#101700

Purpose

Cyc1t in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPD-ADH2t (17-21)

Plasmid

#101701

Purpose

ADH2t in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPD-pTEF2 (01-11)

Plasmid

#101685

Purpose

pTEF2 in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPD-PGK1 (01-11)

Plasmid

#101689

Purpose

pPGK1 in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YCe1905 HC_Kan_DmrA_p21

Plasmid

#100685

Purpose

part designed to occupy position 21 of EMMA. Functional category: Dimerization domain

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

DmrA

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1911 HC_Kan_Insulator_FB_p2

Plasmid

#100686

Purpose

part designed to occupy position 22 of EMMA. Functional category: Insulator

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Insulator_FB

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2052 HC_Kan_DmrC_p9

Plasmid

#100693

Purpose

part designed to occupy position 9 of EMMA. Functional category: Dimerization domain

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

DmrC

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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