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pJJB1463

Plasmid

#218624

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1734

Plasmid

#218627

Purpose

Express amorphadiene synthase (ADS)

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

ADS

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1535

Plasmid

#218613

Purpose

Express Pex5

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1536

Plasmid

#218617

Purpose

Express Pex5 and Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1376

Plasmid

#218618

Purpose

Express Pex5, Pex3, Pex19, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1338

Plasmid

#218592

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB207

Plasmid

#218596

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_R252L

Plasmid

#224153

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(R252L) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(R252L)

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

R252L

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_AJ

Plasmid

#224152

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1 with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1 (B0035.5 Nematode)

Tags

LeuGlu-6xHis

Expression

Bacterial

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_D60N

Plasmid

#224154

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(D60N) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(D60N)

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

D60N

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAN008

Plasmid

#220049

Purpose

Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

flag-HA-GFP

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN053

Plasmid

#220047

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA-mCherry

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-jAspSnFR3-mRuby3

Plasmid

#203459

Purpose

jAspSnFR3-mRuby3 in adenoviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

AAV and Adenoviral

Tags

His-tag and mRuby3

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-miniSOX-3xflag

Plasmid

#206378

Purpose

Expresses 3xflag fused mouse miniSOX in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

miniSOX

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3

Plasmid

#203487

Purpose

Mitochondrial directed jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Lentiviral

Tags

Mitochondrial localization signal

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBA791

Plasmid

#186246

Purpose

dnap-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA792

Plasmid

#186247

Purpose

dnap-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Sp(AU)sgRNA-mKO2

Plasmid

#184443

Purpose

sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

Depositor

Rhys Allan

Article

Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

Insert

sgRNA with AU flip, mKO2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHCKAn-yibDp-Q-GRFT

Plasmid

#158748

Purpose

low phosphate yibDp induction of Q-Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

yibDp Q-Griffithsin

Expression

Bacterial

Mutation

M78Q

Promoter

yibD

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoBp-GRFT

Plasmid

#158746

Purpose

low phosphate phoBp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoBp Griffithsin

Expression

Bacterial

Promoter

phoB

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMST-712

Plasmid

#118080

Purpose

pmbfA::cexA::ttrpC

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

CexA

Promoter

pmbfA

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049d

Plasmid

#96862

Purpose

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT049e

Plasmid

#96863

Purpose

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBsaFa

Plasmid

#85104

Purpose

FAD domain of P450 BM3 variant (C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

FADdomain of P450 BM3

Use

Cloning vector

Mutation

C773S/C810S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBsaPro5X

Plasmid

#85100

Purpose

Putidaredoxin fused to Pro5

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin

Use

Cloning vector

Mutation

changed Cysteine 73 to Serine and Cysteine 85 to …

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHPro51

Plasmid

#85097

Purpose

1st Pro5

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Gly-Gly-Gly-Gly-Ser-Pro-Pro-Pro-Pro-Pro

Use

Cloning vector

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp9-2

Plasmid

#73853

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pJK349

Plasmid

#71566

Purpose

Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly(alanylglycine)

Plasmid

#67003

Purpose

Expresses malE-poly(alanylglycine) in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly(alanylglycine)

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c5X-poly-EAK9

Plasmid

#67010

Purpose

Expresses malE-poly-EAK9 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-EAK9

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-Ti2

Plasmid

#59631

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-Ti1

Plasmid

#59630

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
Rabbit NHERF-1

Plasmid

#31644

Depositor

Edward Weinman

Article

Weinman et al J Clin Invest. 1995 May . 95(5):2143-9.

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Expression

Bacterial

Mutation

none

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL11 mutated

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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