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pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGEM cWnt14 (CT#692)

Plasmid

#13947

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Wnt-14 (WNT9A Chicken)

Expression

Bacterial

Mutation

Note that this insert is in the reverse direction…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) cTbx5 (CT#634)

Plasmid

#13963

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCR-Blunt_hM3O

Plasmid

#112638

Purpose

Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hM3O

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Dendra2-PCNA-19-SV40NLS-4

Plasmid

#57735

Purpose

Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTFP1-PCNA-19-SV40NLS-4

Plasmid

#55505

Purpose

Localization: Nucleus, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEM cMyf5 (CT#141)

Plasmid

#13904

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Myf5 (MYF5 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pFRT-TODestRFP_HuC

Plasmid

#65765

Purpose

expresses RFP-tagged HuC in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL3 (ELAVL3 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
RCAS(B) Chfz-7 WT (CT#533)

Plasmid

#13930

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 7 (FZD7 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
mOrange2-PCNA-19-SV40NLS-4

Plasmid

#57971

Purpose

Localization: Nucleus, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
RCAS(B) Chfz-1 WT (CT#535)

Plasmid

#13932

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 1 (FZD1 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
Ad-EEF1D-S133E

Plasmid

#131577

Purpose

Expresses EEF1D with S133E mutation in mammalian cells

Depositor

Haiyan Xu

Insert

Eukaryotic translation elongation factor 1 delta (EEF1D Human)

Use

Adenoviral

Tags

No

Mutation

changed Serine 133 to Glutamic acid

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_mWasabi

Plasmid

#112640

Purpose

Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

mWasabi

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Flu cTbx4 (CT#700)

Plasmid

#13925

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx4 (TBX4 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) Chfz-7 WT (CT#532)

Plasmid

#13929

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 7 (FZD7 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) cTbx5 D1 (CT#636)

Plasmid

#13968

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Mutation

Amino acids 1-274

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) eGFP

Plasmid

#129020

Purpose

Expresses eGFP

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-mCherry

Plasmid

#123215

Purpose

N-terminal mCherry tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-mSA-EGFP-TM

Plasmid

#39863

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin, EGFP, TM helix

Tags

EGFP, FLAG, and HA

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_HuD

Plasmid

#65760

Purpose

constitutively expresses FLAG-HA-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pBS mPrx1 (CT#640)

Plasmid

#13961

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 promoter (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_HuD

Plasmid

#65757

Purpose

expresses FLAGHA-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_HuB

Plasmid

#65755

Purpose

expresses FLAGHA-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Su(fu) (CT#99)

Plasmid

#13856

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

GST

Expression

Bacterial

Mutation

Nco I fragment from full length; ~2kbp- ATG to st…

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) ZKSCAN1 Sense

Plasmid

#60631

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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Degron-KI donor backbone

Plasmid

#65483

Purpose

Donor backbone vector to clone left and right HR arms in for Degron-KI application

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Type

Empty backbone

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestGFP_HuD

Plasmid

#65763

Purpose

expresses GFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestRFP_HuD

Plasmid

#65766

Purpose

expresses RFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Expression

Mammalian

Mutation

V23M mutation in RFP

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-DestFLAGHA_HuB

Plasmid

#65758

Purpose

constitutively expresses FLAG-HA-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestGFP_HuB

Plasmid

#65761

Purpose

expresses GFP-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Monomer NH_V2

Plasmid

#37527

Purpose

template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

Monomer NH

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pMT-TAK1-V5-His

Plasmid

#63753

Purpose

Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

Depositor

Cyril Gueydan

Insert

TGF-β activated kinase 1 (Tak1 Fly)

Tags

V5-His

Expression

Insect

Promoter

pMT

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_PDZ2

Plasmid

#59735

Purpose

expresses PDZ2 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

GST and thrombin clvg

Expression

Bacterial

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only
GFP Cav2.2 HA L702P pcDNA3

Plasmid

#206056

Purpose

expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

Depositor

Annette Dolphin

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Mutation

L702P

Promoter

CMV

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS mScx (CT#404)

Plasmid

#13958

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (Scx Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
JMJD2C

Plasmid

#39070

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD2C (KDM4C Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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