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We narrowed to 15,946 results for: LEA

Showing: 8001 - 8050 of 15946 results

  13. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid
    #226507
    Purpose
    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain
    Depositor
    Fumio Sakane, Chiaki Murakami
    Article
    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    Three tandem FLAG epitope tag and enterokinase cl…
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    226507-plasmid-map-sequence-id-457149

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  15. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220609
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220609-plasmid-map-sequence-id-441116

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  18. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid
    #127342
    Purpose
    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.
    Insert
    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Expression
    Mutation
    Base pairs 1-1554 of transgene are codon optimize…
    Promoter
    None
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    127342-plasmid-map-sequence-id-246546

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  20. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  21. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid
    #217807
    Purpose
    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217807-plasmid-map-sequence-id-432906

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  22. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid
    #217796
    Purpose
    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217796-plasmid-map-sequence-id-432863

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  23. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  27. pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

    Plasmid
    #210505
    Purpose
    expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    210505-plasmid-map-sequence-id-422559

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  28. pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

    Plasmid
    #210508
    Purpose
    expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
    Use
    Tags
    GB1
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210508-plasmid-map-sequence-id-415564

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  29. pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

    Plasmid
    #210516
    Purpose
    expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210516-plasmid-map-sequence-id-415570

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  30. VC-Y705F+K49R-STAT3

    Plasmid
    #203721
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Use
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K49R substitutions
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203721-plasmid-map-sequence-id-401591

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  31. VC-Y705F+K685R-STAT3

    Plasmid
    #203722
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Use
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K685R substitutions
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203722-plasmid-map-sequence-id-401596

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  34. pcDNA4/TO-bio-myc-cCE

    Plasmid
    #112711
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    HIV Rev nuclear localization signal (NES), Stop c…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV with Tet repressor binding sites
    Available Since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    112711-plasmid-map-sequence-id-217051

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  35. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid
    #202530
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202530-plasmid-map-sequence-id-406370

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  37. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  38. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

    Plasmid
    #196895
    Purpose
    Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    2xHA-CAMSAP3 (Camsap3 Mouse)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196895-plasmid-map-sequence-id-392230

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  39. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196855
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196855-plasmid-map-sequence-id-391813

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  40. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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  43. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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  44. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  46. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid
    #185705
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS
    Depositor
    Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    anCas(PDCA)
    Use
    Tags
    6xHis-NLS and NLS
    Expression
    Mammalian
    Mutation
    optimized for human codon usage
    Promoter
    CMV and T7
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185705-plasmid-map-sequence-id-363631

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  50. pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6

    Plasmid
    #175820
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 3 / MOS6, lacks start ATG and includes stop codon for N-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Article
    Ludke et al Plant J. 2021 Feb;105(4):994-1009. doi:
    Insert
    IMPORTIN ALPHA ISOFORM 3 (MOS6 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175820-plasmid-map-sequence-id-346168

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Showing: 8001 - 8050 of 15946 results