We narrowed to 28,931 results for: CAL;

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Mito-LSSmGFP

Plasmid

#186536

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GB4080

Plasmid

#215249

Purpose

Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

SF-EGFP-p19-LUZ-H3H-HISPS-CPH

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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EB3-mTurquoise2

Plasmid

#98825

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Tags

His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-N1

Plasmid

#54803

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pCMV:: NES-FKBP-iLID-LRP6c

Plasmid

#221774

Purpose

Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: NES-FKBP-iLID-LRP6c

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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AX53_pS.Donor.S1.TS

Plasmid

#100290

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vinculin_TurboID_Venus

Plasmid

#200272

Purpose

Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Expression

Mammalian

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL-Vinculin_TurboID_Venus

Plasmid

#200212

Purpose

Lentivirus vector expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Use

Lentiviral

Promoter

T7

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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EB3-3xmTq2-N1

Plasmid

#112929

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

3x mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AY42_pS.Donor.R5

Plasmid

#100291

Purpose

CCR5-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTS2386-Tier1-PhCMV-GCaMP6s

Plasmid

#169519

Purpose

Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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JB20

Plasmid

#128912

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab11AQL-BirA*-HA-MAO (RAB11A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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