We narrowed to 3,645 results for: MAK;

1. (R42A) Cyclin B1-GFP (62)

   Plasmid

   #61849

   Purpose

   mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

   Depositor

   Jonathon Pines

   Article

   Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

   Insert

   cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Mammalian

   Mutation

   V5A R42A

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAID 1.1 N-T2A-Bsr

   Plasmid

   #105985

   Purpose

   To make an auxin inducible degron mutant

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

   Insert

   T2A-Bsr
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCk2_spacer

   Plasmid

   #136072

   Purpose

   Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. modRNAc0-Cas9

   Plasmid

   #170180

   Purpose

   IVT template plasmid for making modified Cas9 RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9
   

   Use

   Ivt template plasmid

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAID 1.1C-T2A-Bsr

   Plasmid

   #105986

   Purpose

   To make an auxin inducible degron mutant

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

   Insert

   T2A-Bsr
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCk1_spacer

   Plasmid

   #136071

   Purpose

   Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

   Plasmid

   #221361

   Purpose

   Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pB-CAG-TEVp-IRES-mCherry

   Plasmid

   #174377

   Purpose

   For making TEVP cell line with hypBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   TEV Protease
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV GG hUbV-EGFP

   Plasmid

   #216161

   Purpose

   Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CaP2AIII_pK18

    Plasmid

    #153421

    Purpose

    Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha constant region (TRAC*02)-P2A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXC13(human)-pET

    Plasmid

    #8679

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLLX13

    Plasmid

    #79825

    Purpose

    Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

    Depositor

    Cammie Lesser, Stephen Lory

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Yeast and bacteria shuttle vector, contains origi…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYIAL31

    Plasmid

    #60807

    Purpose

    used to make mutations in yeast POL1

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    Pol1 (POL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal part only

    Promoter

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-HD118

    Plasmid

    #172655

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD118A/pegRNA-HD118B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pLLX8

    Plasmid

    #79838

    Purpose

    Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

    Depositor

    Cammie Lesser, Stephen Lory

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Contains yeast counter selection gene, cyh2 and b…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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