We narrowed to 24,857 results for: nes

1. pCMX/cyclinB1 (17)

   Plasmid

   #26060

   Depositor

   Jonathon Pines

   Article

   Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-luxKate

   Plasmid

   #229951

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330A_dCas9-1x7

   Plasmid

   #63601

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-luxKOk

   Plasmid

   #229949

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKOk
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB07

   Plasmid

   #190481

   Purpose

   Targeting plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Upstream & Downstream pyrE deletion region
   gRNA
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330S-3

   Plasmid

   #58779

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDR FLIP43 GPS1

   Plasmid

   #101084

   Purpose

   GA biosensor expression plasmid

   Depositor

   Alexander Jones, Wolf Frommer

   Article

   Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

   Insert

   GPS1
   

   Use

   Tags

   6HIS and cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST HP1 alpha

   Plasmid

   #24074

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 alpha (CBX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAureus-TnCAM

    Plasmid

    #199807

    Purpose

    vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

    Depositor

    Steve Salipante

    Article

    Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

    Insert

    transposon cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Thomas Pollard, Julien Berro

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-EF1a-BbTagBY

    Plasmid

    #45185

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSWEET-bgaB

    Plasmid

    #210272

    Purpose

    Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

    Depositor

    Eric Brown

    Article

    Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

    Insert

    bgaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PxylA

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST HP1 gamma

    Plasmid

    #24076

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMX/cyclinB1-GFP (27)

    Plasmid

    #26061

    Depositor

    Jonathon Pines

    Article

    Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p5'Entry-lck-7.4kb

    Plasmid

    #58891

    Purpose

    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 1874th nucle…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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