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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,908 results for: pcDNA 3.1

Showing: 3201 - 3220 of 5908 results

  1. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74224-plasmid-map-sequence-id-238509

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  2. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  8. SnFR-gamma2

    Plasmid
    #165495
    Purpose
    Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.
    Depositor
    Andrew Plested
    Article
    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755
    Insert
    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165495-plasmid-map-sequence-id-327723

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  14. Emi LC

    Plasmid
    #113666
    Purpose
    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.
    Depositor
    Robert Peters
    Article
    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1
    Insert
    Emicizumab light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    113666-plasmid-map-sequence-id-225230

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  17. Triple mutant

    Plasmid
    #63719
    Purpose
    Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    Triple mutant
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. CC2

    Plasmid
    #63714
    Purpose
    Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q mutations fused to super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    CC2
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    UTS2R (UTS2R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213386-plasmid-map-sequence-id-430893

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Showing: 3201 - 3220 of 5908 results