We narrowed to 39,346 results for: Nes;

1. pX330S-5

   Plasmid

   #58781

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSIM24 KanR

   Plasmid

   #67583

   Purpose

   Plant Gene Expression Vector

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   M24

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Orange_CaMBI_300

   Plasmid

   #124095

   Purpose

   orange calcium-modulated bioluminescent indicator, 300nM

   Depositor

   Michael Lin

   Article

   Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

   Insert

   Orange CaMBI 300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330S-6

   Plasmid

   #58782

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-cdc2-AF (718)

   Plasmid

   #39872

   Depositor

   Jonathon Pines

   Article

   Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

   Insert

   Cdc2 (CDK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AF = T14A, Y15F

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. GeNL(Ca2+)_520/pcDNA3

   Plasmid

   #85204

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_520
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YeNL_pRSETb

   Plasmid

   #89520

   Purpose

   Yellow color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX302 LacZ-V5 puro

   Plasmid

   #98579

   Purpose

   Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   2x V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-13

    Plasmid

    #119992

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330A-1x4

    Plasmid

    #58768

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Blue_CaMBI

    Plasmid

    #124097

    Purpose

    Blue calcium-modulated bioluminescent indicator

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Blue CaMBI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Green_CaMBI

    Plasmid

    #124096

    Purpose

    Green calcium-modulated bioluminescent indicator

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Green CaMBI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GeNL(Ca2+)_60/pcDNA3

    Plasmid

    #85207

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p5'Entry-lck-5.5kb

    Plasmid

    #58890

    Purpose

    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 13th nucleot…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PEP109E

    Plasmid

    #97395

    Purpose

    binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    cytosol

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSGV Hu Acceptor PGK-NGFR

    Plasmid

    #64270

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD3

    Plasmid

    #118043

    Purpose

    GoldenBraid2.0 Universal Part Domesticator #3

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge