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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,269 results for: ef1;

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Showing: 3221 - 3240 of 9269 results

  2. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  3. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  6. pMK1254

    Plasmid
    #133057
    Purpose
    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133057-plasmid-map-sequence-id-267979

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  9. pMK1253

    Plasmid
    #133058
    Purpose
    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mClover2
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133058-plasmid-map-sequence-id-267978

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  10. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO Elongation factor 1A
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

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  19. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-mCherry
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211782-plasmid-map-sequence-id-417156

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  20. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-DNMT3A
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211785-plasmid-map-sequence-id-417372

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Showing: 3221 - 3240 of 9269 results