164,811 results

1. pcDNA3-ULK2

   Plasmid

   #97075

   Purpose

   Expresses ULK2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

   Insert

   ULK2 (ULK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV Blast empty (w263-1)

   Plasmid

   #17486

   Purpose

   3rd gen lentiviral empty control vector, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2 TDP-43 NLS1 YFP

   Plasmid

   #84912

   Purpose

   Mammalian expresion of TDP-43 NLS1 YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A

   Promoter

   CMV

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-APOBEC1-YTH

   Plasmid

   #178949

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTH-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-PEmax for IVT

   Plasmid

   #178113

   Purpose

   Template for in vitro transcription of PEmax

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Detailed in manuscript

   Promoter

   T7 (inactivated)

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H187

   Plasmid

   #170348

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Ubiquitin-K27

   Plasmid

   #22902

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K27 only, all other lysines mutated to arginines

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB2-mScarlet-I

   Plasmid

   #104007

   Purpose

   Plasmid encoding mScarlet-I

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mScarlet-I
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myosin-IIA-GFP

    Plasmid

    #38297

    Depositor

    Matthew Krummel

    Article

    Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

    Insert

    Myosin II A (Myh9 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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