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Showing: 4861 - 4890 of 8424 results

  1. F3 Neo* F3

    Plasmid
    #140235
    Purpose
    F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Flp/ frt
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140235-plasmid-map-sequence-id-316186

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  2. pREP41xV5_ccdB

    Plasmid
    #79017
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79017-plasmid-map-sequence-id-151963

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  3. pREP42xV5_ccdB

    Plasmid
    #79018
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79018-plasmid-map-sequence-id-147775

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  4. 4LDG

    Plasmid
    #60090
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd4_370:MAC04H-G03:C228753
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  6. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid
    #233036
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233036-plasmid-map-sequence-id-465997

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  7. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  8. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Use
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  10. AAV-DIO-TVA-2A-oG

    Plasmid
    #178431
    Purpose
    Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178431-plasmid-map-sequence-id-354735

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  14. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162257-plasmid-map-sequence-id-319826

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  15. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

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  16. attB Neo* attP

    Plasmid
    #138412
    Purpose
    attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Att/phic31
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138412-plasmid-map-sequence-id-316184

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  18. mT2A-td-sfGFP-FNF

    Plasmid
    #182339
    Purpose
    Recombineering cassette plasmid for T2A-td-sfGFP-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-td-sfGFP-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182339-plasmid-map-sequence-id-358195

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  20. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109059-plasmid-map-sequence-id-209551

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  21. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109058-plasmid-map-sequence-id-209548

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  23. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid
    #233035
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233035-plasmid-map-sequence-id-467423

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  24. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

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  25. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  26. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid
    #182338
    Purpose
    Template plasmid for PCR amplification of initial recombineering cassette
    Depositor
    Mario Capecchi
    Insert
    BSD
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182338-plasmid-map-sequence-id-358191

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Showing: 4861 - 4890 of 8424 results