We narrowed to 15,991 results for: LEA

1. pBAD24-fis

   Plasmid

   #51557

   Purpose

   E. coli fis ORF cloned into pBAD24 expression vector

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   FIS (fis E. coli)
   

   Expression

   Bacterial

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-Pol-IIΔ

   Plasmid

   #35176

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the entire C-terminal domain (repeats…

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EF05J

   Plasmid

   #42941

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Negative control Functionally Dead Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   ORF1 R261A, R262A

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pX330A-1x6

   Plasmid

   #58770

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-AsCas12f1

   Plasmid

   #171614

   Purpose

   AsCas12f1-based Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1 and sgRNA_v1
   

   Expression

   Mammalian

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-Aurora A

   Plasmid

   #198402

   Purpose

   Expression of EGFP-tagged Aurora A in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Aurora A
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMCP-RecT

   Plasmid

   #183827

   Purpose

   pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   MCP-RecT_NLS
   

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF6.mCherry-TSG101

    Plasmid

    #38318

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    TSG101 (TSG101 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

    Plasmid

    #205277

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    C310R/D487K/T513K

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAMBgfp

    Plasmid

    #206130

    Purpose

    This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

    Depositor

    Anne Osbourn

    Article

    Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

    Insert

    hygromycin resistance gene and the Promoter of ToxA+SGFP
    

    Use

    Binary vector for expression of sgfp in filamento…

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_SUMO_AcCas12n

    Plasmid

    #203808

    Purpose

    pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    SUMO_AcCas12n
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. stathmin-GFP

    Plasmid

    #86782

    Purpose

    Mammalian expression of stathmin-GFP

    Depositor

    Lynne Cassimeris

    Article

    Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

    Insert

    stathmin (STMN1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-LMO-IRES-EGFP

    Plasmid

    #178309

    Purpose

    Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    Lactate 2-monooxygenase
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Reb_206 aka PAS629

    Plasmid

    #71234

    Purpose

    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    mNeonGreen to second copy RebB, soluble mCherry a…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-Nup88

    Plasmid

    #64283

    Purpose

    GFP-tagged mammalian expression of full-length Nup88

    Depositor

    Birthe Fahrenkrog

    Article

    Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

    Insert

    Nup88 (NUP88 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only