We narrowed to 14,077 results for: cas9

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

   Plasmid

   #118202

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

   Plasmid

   #107301

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
   

   Use

   CRISPR

   Tags

   FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

   Plasmid

   #101182

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

   Plasmid

   #101183

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   M694A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

   Plasmid

   #101180

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

    Plasmid

    #69231

    Purpose

    Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 TALE
    

    Use

    CRISPR

    Tags

    2X NLS and NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

    Plasmid

    #237890

    Purpose

    Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE and U6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid

    #188902

    Purpose

    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-KRAB(Kox1)-MeCP2
    

    Use

    Lentiviral

    Tags

    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

    Promoter

    SFFV

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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