We narrowed to 6,537 results for: poly

1. CDS_GUS

   Plasmid

   #136084

   Purpose

   For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS_GUS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. CDS12_eGFP-linker

   Plasmid

   #136086

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_eGFP(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDS12_mScarletI-linker

   Plasmid

   #136088

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mScarletI(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pMK110

   Plasmid

   #99535

   Purpose

   contains bgl operon sequence for E. coli in vitro transcription template

   Depositor

   Robert Landick

   Article

   Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

   Insert

   E. coli bgl antisense region containing bglG
   

   Use

   Tags

   None

   Expression

   Mutation

   Promoter

   lambda Pr promoter used for in vitro transcriptio…

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046802

   Plasmid

   #145683

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGEX-5X-1-DJ1-D149A

   Plasmid

   #29351

   Depositor

   Mark Cookson

   Article

   Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Asp149Ala

   Promoter

   Available since

   March 21, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pJZ001

   Plasmid

   #120230

   Purpose

   Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   intB13 integrase casette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   bla-g717a (amp resistance gene)

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FT1

   Plasmid

   #31435

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Fused Toes Homolog (AKTIP Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pmCherry-FKBP12-C1-DH

   Plasmid

   #67901

   Purpose

   Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   mCherry-FKBP12-DH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac K-Flag M33

    Plasmid

    #1992

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

11. psicheck-2-Sfxn3

    Plasmid

    #48169

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of SFXN3 (SFXN3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET:CCCT:GFP

    Plasmid

    #60738

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJKW1847

    Plasmid

    #174004

    Purpose

    Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP94D108
    ATR1 (ATR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-5X-1-DJ1-M26I

    Plasmid

    #29353

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Met26Ile

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

    Plasmid

    #24606

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, Y459A

    Promoter

    Available since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pFastBac K-M33-Flag

    Plasmid

    #1993

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pIS1 Cyclin D2 short UTR

    Plasmid

    #21644

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 short UTR (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A

    Plasmid

    #24607

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, Y459A, C716A

    Promoter

    Available since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pMH-HT_ygiF-FL

    Plasmid

    #127795

    Purpose

    Expresses full-length ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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