We narrowed to 7,255 results for: 11

1. GW1-mito-roGFP1-mApple

   Plasmid

   #107218

   Purpose

   Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   roGFP1-mApple
   

   Use

   Tags

   4xCox8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

   Plasmid

   #132683

   Purpose

   AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Carnosine dipeptidase 1 (Cndp1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEYFP-hDF-C2A

   Plasmid

   #72582

   Purpose

   human dysferlin C2A domain tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin C2A domain (DYSF Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C2A domain

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK3_010

   Plasmid

   #123725

   Purpose

   Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   TetOn3G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3045(gRNA XI-3)

   Plasmid

   #73287

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_speG_Cterm_His

   Plasmid

   #220483

   Purpose

   heterologous expression for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   speG
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-YdLight1

   Plasmid

   #125705

   Purpose

   Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Insert

   YdLight1
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PVT1-Tol2

   Plasmid

   #113381

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   PVT1-enhancer (PVT1 Human)
   

   Use

   Tol2 reporter

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. STARR-seq luciferase validation vector_SCP1_empty

    Plasmid

    #99299

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. GW1-mito-mCherry-roGFP2

    Plasmid

    #107220

    Purpose

    Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    mCherry-roGFP2
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MTK8b_004

    Plasmid

    #123892

    Purpose

    Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' HIV LTR (3rd gen.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cx43K258stop-msfGFP

    Plasmid

    #69023

    Purpose

    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Mutation

    delete codons 258-381 and fuse in frame to monome…

    Promoter

    CMV

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMIY-mETV3-Flag

    Plasmid

    #34579

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pBJG1 T7-8xHis-OGT D554N

    Plasmid

    #154279

    Purpose

    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Aspartate 554 changed to Asparagine- bacterial co…

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK7_014

    Plasmid

    #123881

    Purpose

    Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3' HIV LTR (3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK3b_031

    Plasmid

    #123809

    Purpose

    Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    KRAB:meCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge