We narrowed to 4,744 results for: NAP

1. GABA(A) receptor subunit a1SE

   Plasmid

   #49168

   Purpose

   pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Tija Jacob, Stephen Moss

   Article

   Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

   Insert

   GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
   

   Use

   Tags

   myc and pHGFP (pHIuorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. DNA-PKcs PQR>ala

   Plasmid

   #83319

   Purpose

   human DNA-PKcs PQR>ala

   Depositor

   Katheryn Meek

   Article

   Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 phosphorylation sites in the PQR cluster substi…

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-Jaws-GFP-ER2

   Plasmid

   #65016

   Purpose

   AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-GFP-ER2
   

   Use

   AAV

   Tags

   ER2 and GFP

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   human synapsin

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. DNA-PKcs ABCDE>A

   Plasmid

   #83318

   Purpose

   human DNA-PKcs/ABCDE>ala

   Depositor

   Katheryn Meek

   Article

   Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   6 phosphorylation sites in the ABCDE cluster subs…

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET iGluSnFR3 v82

   Plasmid

   #175187

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v82
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

   Plasmid

   #111389

   Purpose

   AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   ChETA-mRuby2
   W3SL
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   W3SL is built from a shortened WPRE, an upstream …

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+/-FLI1-C‐(K)‐DYK

   Plasmid

   #129497

   Purpose

   Mammalian expression of human FLI1

   Depositor

   Marjorie Brand

   Article

   Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

   Plasmid

   #165082

   Purpose

   gRNA 1 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIRTS-control

    Plasmid

    #213788

    Purpose

    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

    Insert

    CIRTS-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHAGE-APPSw;T719N

    Plasmid

    #177094

    Purpose

    For lentiviral transduction of APPsw; T719N

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    APPsw;T719N (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T719N, K651N and M652L (please see depositor comm…

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-TO-RPB1-His WT CRres si2,4R

    Plasmid

    #139404

    Purpose

    Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV, TRex

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER-21-HOXA10

    Plasmid

    #97042

    Purpose

    Expresses HOXA10 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Homeobox A10 (HOXA10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

18. CamKII-ADAM23-pHluorin

    Plasmid

    #185539

    Purpose

    Expresses ADAM23-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    ADAM23 (Adam23 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1a-extGluc-mKate-NGFR

    Plasmid

    #104831

    Purpose

    extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    mKate
    NGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

    Plasmid

    #111388

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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