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pSUMO R106L-CPTP

Plasmid

#170737

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

R106L-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

R106L

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO K60A-CPTP

Plasmid

#170736

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

K60A-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

K60A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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csd6_17-330/pET28b(+) N-tag

Plasmid

#71353

Purpose

csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_E110A/pET28b(+) N-tag

Plasmid

#71354

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Glutamic acid 110 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y133A/pET28b(+) N-tag

Plasmid

#71356

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 133 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_C176A/pET28b(+) N-tag

Plasmid

#71360

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Cysteine 176 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H160A/pET28b(+) N-tag

Plasmid

#71359

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 160 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H155A/pET28b(+) N-tag

Plasmid

#71357

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 155 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y132A/pET28b(+) N-tag

Plasmid

#71355

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 132 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5b HA Irx5 deltaHD

Plasmid

#24997

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletes entire HD (aa 112-177)

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Rat, Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSecUAG-Evol2

Plasmid

#163148

Purpose

Expresses machinery necessary for selenocysteine incorporation at UAG codons

Depositor

Dieter Söll

Article

Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

Inserts

allo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase

Expression

Bacterial

Mutation

Cysteine 32 switched to Selenocysteine (UAG), Cys…

Promoter

EM7, araBAD, native As SelD promoter, and native …

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK3-CR

Plasmid

#229422

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK3-CR (MARK3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation CRISPR resistant

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95H_mCherry

Plasmid

#84023

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95H

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-3

Plasmid

#229442

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-3 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF1.0

Plasmid

#208661

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-B

Plasmid

#66188

Purpose

expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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