We narrowed to 4,850 results for: AAT

1. eGFP-SUN1

   Plasmid

   #213515

   Purpose

   Expresses human eGFP-SUN1 in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   SUN1 (SUN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SUN1 has a silent mutation on its gene (nucleotid…

   Promoter

   CMV

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAf-CRISPR-yA

   Plasmid

   #191015

   Purpose

   CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

   Insert

   yA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAE1 G8.3 gRNA

   Plasmid

   #90777

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NAE1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NAE1 (Guide Designation G8.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Anillin

   Plasmid

   #68027

   Purpose

   Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

   Depositor

   Michael Glotzer

   Article

   Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

   Insert

   Anillin (ANLN Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations at 798 5'-TGCC TCTTTGAATAAA…

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-sgMyc_1

   Plasmid

   #138324

   Purpose

   Express guide RNA 2 for mouse Myc

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   cMyc sgRNA (Myc Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-Tet-Puro-shIAPEz_2

   Plasmid

   #185017

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_IAPEz
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-Tet-Puro-shIAPEz_1

   Plasmid

   #185016

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_IAPEz
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_TRE_Ncad-P2A-GFPlig

   Plasmid

   #133806

   Purpose

   inducible expression of Ncad and membrane-tethered GFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 2 (CDH2) (Cdh2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Mary F Roberts, Anne Gershenson

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. RRM1 E2.3 gRNA

    Plasmid

    #90881

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-AAV-sh[SNCA]

    Plasmid

    #75437

    Purpose

    knockdown alpha-synuclein in rat neurons

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    snca (Snca Rat)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. tet_pLKO.1_puro_shLuc

    Plasmid

    #136587

    Purpose

    Expresses an inducible short hairpin targeting firefly luciferase sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-LyzC-RacFRET-U6ac-ctrl guides

    Plasmid

    #168247

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATP1A1_G3_Dual_sgRNA

    Plasmid

    #173202

    Purpose

    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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