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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,876 results for: Coli

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Showing: 3301 - 3320 of 9876 results

  8. pKB235.4

    Plasmid
    #170026
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
    170026-plasmid-map-sequence-id-335946

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  9. pKB236.1

    Plasmid
    #170028
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP Δ C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
    170028-plasmid-map-sequence-id-335958

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  10. pKB236.2

    Plasmid
    #170029
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
    170029-plasmid-map-sequence-id-335296

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  11. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available Since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  12. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  13. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  14. PCDF Bravo-PotH-OL1del

    Plasmid
    #216749
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 1 (OL1) with the sequence of [FKISLAEM…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216749-plasmid-map-sequence-id-434430

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  15. PCDF Bravo-PotH-OL2del

    Plasmid
    #216750
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216750-plasmid-map-sequence-id-434427

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  16. PCDF Bravo-PotH-OL3del

    Plasmid
    #216751
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 3 (OL3) with the sequence of [ELLGGPDS…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216751-plasmid-map-sequence-id-434425

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  17. PCDF Bravo-PotH-OL3/2sub

    Plasmid
    #216752
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216752-plasmid-map-sequence-id-434424

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Showing: 3301 - 3320 of 9876 results