We narrowed to 5,023 results for: puc

1. LI C-D mCherry noATG

   Plasmid

   #54440

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   mCherry without Start codon
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. LI C-D YFP noATG

   Plasmid

   #54437

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   YFP Venus without Start codon
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGF004

   Plasmid

   #48845

   Purpose

   Provides a D-alanine resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   p35S::D-AlanineR::t35S
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition site in 35S promoter re…

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGA002

   Plasmid

   #48813

   Purpose

   Provides the AP3 promoter as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   AP3 promoter
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition site removed by substit…

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CJ47 (U1 snRNA (U1;21d) 3G mutant)

   Plasmid

   #17776

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   U1 snRNA (U1;21d) (snRNA:U1:21D Fly)
   

   Use

   Tags

   Expression

   Mutation

   3G mutant

   Promoter

   Available since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pILGFP4G

   Plasmid

   #104502

   Purpose

   Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   ScPGM2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP4K

   Plasmid

   #104506

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpGAL1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP4O

   Plasmid

   #104510

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpdGAL2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP4P

   Plasmid

   #104511

   Purpose

   Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SptGAL2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PECAM1-mRuby3-T2A-secNluc

    Plasmid

    #235607

    Purpose

    Donor plasmid for knock-in of mRuby3-T2A-secNluc into the human PECAM1 locus

    Depositor

    Kai Wang

    Article

    Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

    Insert

    PECAM1 homologous recombination arms with PuroR drug selection cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK305

    Plasmid

    #66812

    Purpose

    IVT vector for Fluc mRNA

    Depositor

    Ron Weiss

    Article

    Andries et al J Control Release. 2015 Sep 2. pii: S016

    Insert

    Firefly luciferase
    

    Use

    Luciferase; Mrna ivt

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. EcoHIV-mScarlet

    Plasmid

    #235598

    Purpose

    EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

    Depositor

    Michael Shtutman

    Article

    Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

    Insert

    NL4-3 EcoHIV mScarlet
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

    Plasmid

    #203770

    Purpose

    For production of template RNA for use in PRINT, mCherry under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for mCherry
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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